Leucine regulates slow‐twitch muscle fibers expression and mitochondrial function by Sirt1/AMPK signaling in porcine skeletal muscle satellite cells

• DOI: 10.1111/asj.13146
• Publication Date: 2018-12-07T04:49:56Z
• Authors: Xiaoling Chen; Lu Xiang; Gang Jia; Guangmang Liu; Hua Zhao; Zhiqing Huang
• Keywords: AMP-Activated Protein Kinase

Abstract A previous study demonstrated that leucine upregulates the slow myosin heavy chain mRNA expression in C2C12 cells. However, role of slow‐twitch muscle fibers and mitochondrial function porcine skeletal satellite cells as well its mechanism remain unclear. In this study, cultured differentiation medium were treated with 2 mM for 3 days. Sirt1 inhibitor EX 527, AMPK compound C, α1 si RNA used to examine underlying mechanism. Here we showed increased function‐related gene expression, succinic dehydrogenase ( SDH ) malate MDH activities. Moreover, protein levels phospho‐ . We also found , or 527 attenuated positive effect on expression. Finally, was required leucine‐induced activation. Our results provide, first time, evidence induces improves through Sirt1/ signaling pathway