Heat shock protein 70 potentiates interferon alpha production by plasmacytoid dendritic cells: relevance for cutaneous lupus and vitiligo pathogenesis

• DOI: 10.1111/bjd.15550
• Publication Date: 2017-04-05T22:25:00Z
• Authors: C. Jacquemin; J. Rambert; S. Guillet; D. Thiolat; N. Boukhedouni; M.‐S. Doutre; A.‐S. Darrigade; K. Ezzedine; P. Blanco; A. Taieb; K. Boniface; J. Seneschal
• Keywords: Vitiligo; TLR7; TLR9

Plasmacytoid dendritic cells (pDCs) are a subset of specialized in the production type I interferon (IFN-α/β) and involved various cutaneous inflammatory autoimmune disorders, such as lupus erythematosus (CLE) vitiligo. Heat shock proteins (HSPs) molecular chaperones essential for maintaining cellular functions, but they can act danger signal during inflammation.To decipher role HSP70 IFN-α by pDCs CLE vitiligo.Expression CD123+ was analysed immunohistochemistry or immunofluorescence vitiligo skin samples. Flow cytometry performed to analyse expression receptors, activation markers on DNA uptake presence HSP70. The impact DNA-induced secretion evaluated enzyme-linked immunosorbent assay (ELISA). effect chemokine (C-X-C motif) ligand 9 (CXCL9)/10 gene protein keratinocytes determined real-time polymerase chain reaction ELISA.Infiltration progressive primarily located epidermis, close expressing In vitro experiments revealed that receptor Lox-1 (lectin-like oxidized low-density lipoprotein-receptor-1) were able aggregate Exogenous induced increased exogenous DNA. Furthermore, potentiated pDCs. Finally, CXCL9 CXCL10 keratinocytes.These data demonstrate interaction between is prerequisite enhancement production, could be an interesting target.