SOX12: a novel potential target for acute myeloid leukaemia

• DOI: 10.1111/bjh.14425
• Publication Date: 2016-11-18T09:23:29Z
• Authors: Haixia Wan; Jiayi Cai; Fangyuan Chen; Jianyi Zhu; Jihua Zhong; Hua Zhong
• Keywords: Myeloid leukaemia

The role of SRY-related high-mobility-group box (SOX) 12 in leukaemia progression and haematopoiesis remains elusive. This study aimed to examine the expression function SOX12 acute myeloid (AML) using human samples cell line THP1. Mononuclear cells were isolated from bone marrow AML patients healthy donors. haematopoietic was evaluated by reverse transcription polymerase chain reaction (RT-PCR). short hairpin RNAs (shRNAs) transduced into THP1 cells, gene knockdown confirmed quantitative RT-PCR Western blot analysis. preferentially expressed CD34+ patients. with shRNAs exhibited significantly reduced proliferation. had no effect on apoptosis, but it induced cycle arrest at G1 phase number colonies. primary reconstituted non-obese diabetic-severe combined immunodeficient (NOD/SCID) mice, their numbers 6-12 weeks after transplantation. mRNA protein levels β-catenin diminished following knockdown, accompanied a decrease TCF/Wnt activity. may be involved regulating then interfering pathway, which target for AML.