Methyl jasmonate sensitizes human bladder cancer cells to gambogic acid‐induced apoptosis through down‐regulation of EZH2 expression by miR‐101

• DOI: 10.1111/bph.12501
• Publication Date: 2013-11-06T10:49:15Z
• Authors: Yongjun Wang; Wei Xiang; Miao Wang; Tao Huang; Xingyuan Xiao; Liang Wang; Dan Tao; Liyun Dong; Fuqing Zeng; Guosong Jiang
• Keywords: Gambogic acid; Methyl jasmonate; Viability assay

Background and Purpose Gambogic acid ( GA ) methyl jasmonate MJ are increasingly being recognized as novel natural anticancer compounds. Here, we investigated the antitumour effects of in combination with on human bladder cancer cells. Experimental Approach Cell viability was detected by cell counting kit‐8 assay. apoptosis assessed H oechst 33258 staining flow cytometry. Protein levels were determined immunoblotting expressions mRNA miRNAs RT‐PCR . Differential a group downstream genes identified using microarray analysis. Key Results significantly sensitized cells to ‐induced growth inhibition while sparing normal fibroblasts. enhanced activation caspase‐3 caspase‐9, down‐regulated expression X IAP. Furthermore, treatment induced synergistic enhancer zeste homologue 2 EZH 2) expression, whereas miR ‐101 up‐regulated. Conversely, knockdown restored this decreased suppressed inhibitory effect Microarray analysis showed that closely associated development MJ. In s.c. xenograft mouse model carcinoma, exerted an increased compared alone. Conclusion Implications sensitizes down‐regulating ‐101. Thus, selective anti‐cancer agents could provide strategy for treating cancer.