Involvement of the orexin/hypocretin system in the pharmacological effects induced by Δ9‐tetrahydrocannabinol

• DOI: 10.1111/bph.13440
• Publication Date: 2016-01-22T14:02:07Z
• Authors: África Flores; Marina Julià‐Hern...; Rafael Maldonado; Fernando Berrendero
• Keywords: Anxiogenic

Background and Purpose Anatomical, biochemical pharmacological evidence suggest the existence of a crosstalk between orexinergic endocannabinoid systems. While orexin receptor 1 (OX receptor) modulates reinforcing properties cannabinoids, participation orexins in acute effects Δ 9 ‐tetrahydrocannabinol (THC) remains unexplored. Experimental Approach We assessed possible role THC‐induced hypolocomotion, hypothermia, antinociception, anxiolytic‐ anxiogenic‐like memory impairment. Selective OX 2 antagonists knockout (KO) mice as well prepro‐orexin (PPO) KO were used genetic approaches. CB levels control PPO evaluated by immunoblot analysis. The expression c‐Fos after THC treatment was analysed several brain areas wild‐type lacking gene. Key Results supraspinal antinociception anxiolytic‐like induced modulated through signalling. receptors did not seem to be involved these responses. No differences found mice. increase reduced central amygdala, medial preoptic area lateral septum mutant Conclusions Implications Our results provide new findings further clarify interaction cannabinoids. are differently implicated