Potent effects of dioscin against pancreatic cancer via miR‐149‐3P‐mediated inhibition of the Akt1 signalling pathway

• DOI: 10.1111/bph.13718
• Publication Date: 2017-01-17T19:20:03Z
• Authors: Lingling Si; Lina Xu; Lianhong Yin; Yan Qi; Xu Han; Youwei Xu; Yanyan Zhao; Kexin Liu; Jinyong Peng
• Keywords: Nude mouse; Viability assay

Background and Purpose The aim of the present study was to investigate effects possible underlying mechanisms dioscin against pancreatic cancer in vitro vivo . Experimental Approach In actions on viability ASPC‐1 PANC‐1 cells, suppress tumour growth cell xenografts nude mice were assessed. addition, microRNA microarray analysis determined which microRNAs affected by dioscin. elucidated terms Akt1 other proteins related aopoptosis. Key Results Dioscin markedly induced apoptosis significantly suppressed xenografts, mice. Total 107 with differential changes found, miR‐149‐3P targeted up‐regulated Further studies showed that down‐regulated levels, thus increasing levels Bax, Apaf‐1, cleaved caspase‐3/9, PARP, suppressing Bcl‐2 causing cytochrome c release. an inhibitor siRNA testicular suggested excellent activity via miR‐ 149‐3P‐mediated inhibition signalling pathway. Conclusions Implications Collectively, these findings confirmed potent also provided novel insights into compound as a potential candidate for treatment cancer.