Comparative study of one‐step nucleic acid amplification assay, frozen section, and touch imprint cytology for intraoperative assessment of breast sentinel lymph node in Chinese patients

• DOI: 10.1111/cas.12001
• Publication Date: 2012-08-24T06:57:14Z
• Authors: Yong‐sheng Wang; Tao Ou‐yang; Jiong Wu; Yan‐hui Liu; Xu‐chen Cao; Xiao Sun; Li Fu; Ning Liao; Wen‐tao Yang; Wei‐xia Zhong; Ai‐ping Lu
• Keywords: Histology

Conventional procedures for the intraoperative assessment of breast cancer sentinel lymph nodes (SLNs) are frozen section (FS) and touch imprint cytology (TIC). The one-step nucleic acid amplification (OSNA) assay is a novel molecular technique. aim this study was to evaluate optimal approach by comparing OSNA assay, FS, TIC. Five hundred fifty-two consecutive patients were enroled from five centers in China. SLNs cut into alternating 2 mm blocks. odd blocks tested intraoperatively, even ones assessed postoperative histology (four 4- 6-μm-thick sections taken every 200 μm per block). In addition, histological assessments carried out on 211 FS all 552 Overall performance compared was: accuracy 91.4%; sensitivity 83.7%; specificity 92.9%. higher than (211 patients, 77.6% vs 69.7%; not significant, P = 0.286) significantly TIC (552 83.6% 76.2%; 0.044). When assessing with micrometastases, (17 nodes, 47.1% 23.5%; 0.289) (48 62.5% 35.4%; 0.007). indicated that an accurate rapid it can replace application general medical practice. trial registered as: China Registration Study.China Breast Cancer Clinical Study Group 001c.