Upregulation of sialidase NEU3 in head and neck squamous cell carcinoma associated with lymph node metastasis

• DOI: 10.1111/cas.12810
• Publication Date: 2015-09-07T09:04:51Z
• Authors: Kiyoto Shiga; Kohta Takahashi; Ikuro Sato; Kengo Kato; Shigeru Saijo; Setsuko Moriya; Masahiro Hosono; Taeko Miyagi
• Keywords: Adult; Aged, 80 and over; Male; 0301 basic medicine; Squamous Cell Carcinoma of Head and Neck; Immunoblotting; Neuraminidase; Original Articles; Middle Aged; Real-Time Polymerase Chain Reaction; Transfection; Immunohistochemistry; 3. Good health; 03 medical and health sciences; Head and Neck Neoplasms; Lymphatic Metastasis; Biomarkers, Tumor; Carcinoma, Squamous Cell; Disease Progression; Humans; Female; Chromatography, Thin Layer; RNA, Small Interfering; Aged

Regional lymph node metastasis in head and neck squamous cell carcinoma (HNSCC) is a crucial event for its progression, associated with high rate of mortality. Sialidase, key enzyme the regulation cellular sialic acids through catalyzing initial step degradation glycoproteins glycolipids, has been implicated cancer progression. To facilitate development novel treatments HNSCC, we have investigated whether sialidase involved progression this cancer. We found plasma membrane-associated (NEU3) to be significantly upregulated tumor compared non-tumor tissues; particularly, an increase mRNA levels was metastasis. understand mechanisms, analyzed NEU3-mediated effects on malignant phenotype using HSC-2 SAS cells. NEU3 promoted motility invasion, accompanied by increased expression MMP-9, whereas silencing or activity-null mutant did not. enhanced phosphorylation epidermal growth factor receptor (EGFR), EGFR inhibitor, AG1478, abrogated NEU3-induced MMP9 augmentation. These findings identify as participant HNSCC signaling thus potential target inhibiting EGFR-mediated