MiR‐133b inhibits growth of human gastric cancer cells by silencing pyruvate kinase muscle‐splicer polypyrimidine tract‐binding protein 1

• DOI: 10.1111/cas.13091
• Publication Date: 2016-10-11T17:30:17Z
• Authors: Taro Sugiyama; Kohei Taniguchi; Nobuhisa Matsuhashi; Toshihiro Tajirika; Manabu Futamura; Tomoaki Takai; Yukihiro Akao; Kazuhiro Yoshida
• Keywords: Polypyrimidine tract-binding protein; PKM2; Warburg Effect

The metabolism in tumor cells shifts from oxidative phosphorylation to glycolysis even an aerobic environment. This phenomenon is known as the Warburg effect. effect regulated mainly by polypyrimidine tract‐binding protein 1 ( PTBP ), which a splicer of m RNA for rate‐limiting enzymes glycolysis, pyruvate kinase muscle and 2 PKM 2). In present study, we demonstrated that miR‐133b reduced expression at translational level levels were significantly downregulated gastric cancer clinical samples human cell lines, whereas was upregulated 80% 20 examined. Ectopic knockdown inhibited proliferation through induction autophagy switching isoform 2‐dominant 1‐dominant. growth inhibition partially canceled inhibitor 3‐ MA or reactive oxygen species scavenger N ‐acetylcysteine. These findings indicated acted tumor‐suppressor negative regulation cells.