Downregulation of microRNA‐100/microRNA‐125b is associated with lymph node metastasis in early colorectal cancer with submucosal invasion

• DOI: 10.1111/cas.13152
• Publication Date: 2016-12-29T14:37:31Z
• Authors: Yasuteru Fujino; Shunsaku Takeishi; Kensei Nishida; Koichi Okamoto; Naoki Muguruma; Tetsuo Kimura; Shinji Kitamura; Hiroshi Miyamoto; Akiko Fujimoto; Jun Higashijima; Mitsuo Shimada; Kazuhito Rokutan; Tetsuji Takayama
• Keywords: 0303 health sciences; TOR Serine-Threonine Kinases; Receptors, Somatomedin; X-Linked Inhibitor of Apoptosis Protein; Original Articles; HCT116 Cells; Matrix Metalloproteinases; Receptor, IGF Type 1; 3. Good health; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Lymphatic Metastasis; Humans; RNA Interference; fas Receptor; RNA, Small Interfering; Colorectal Neoplasms; HT29 Cells; Cell Proliferation

A majority of early colorectal cancers ( CRC s) with submucosal invasion undergo surgical operation, despite a very low incidence lymph node metastasis. Our study aimed to identify micro RNA s (mi specifically responsible for metastasis in s. Micro microarray analysis revealed that miR‐100 and miR‐125b expression levels were significantly lower tissues metastases than those without metastases. These results validated by quantitative real‐time PCR larger set clinical samples. The transfection or inhibitor into colon cancer HCT 116 cells increased cell invasion, migration, MMP activity. Conversely, overexpression mimics attenuated all these activities but did not affect growth. To target m s, we undertook gene array miR‐100‐silenced as well negative control cells. Ingenuity Pathway Analysis, TargetScan software analyses, subsequent verification identified mammalian rapamycin (m TOR ) insulin‐like growth factor 1 receptor IGF 1R) direct, Fas X‐linked inhibitor‐of‐apoptosis protein XIAP indirect candidate targets involved Knockdown each si reduced the invasiveness data clearly show downregulation is closely associated through enhancement motility, In particular, may promote upregulating , 1R, Fas, targets. Thus, be novel biomarkers invasion.