Long noncoding RNA SchLAH suppresses metastasis of hepatocellular carcinoma through interacting with fused in sarcoma

• DOI: 10.1111/cas.13200
• Publication Date: 2017-02-14T18:36:40Z
• Authors: Zhouhong Ge; Zhuoan Cheng; Xinrong Yang; Xisong Huo; Ning Wang; Hui Wang; Cun Wang; Dishui Gu; Fangyu Zhao; Ming Yao; Jia Fan; Wenxin Qin
• Keywords: rac1 GTP-Binding Protein; 0301 basic medicine; Mice, Inbred BALB C; Carcinoma, Hepatocellular; Reverse Transcriptase Polymerase Chain Reaction; Blotting, Western; Liver Neoplasms; Transplantation, Heterologous; Mice, Nude; Original Articles; Hep G2 Cells; Prognosis; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Animals; Humans; RNA-Binding Protein FUS; RNA Interference; RNA, Long Noncoding; Neoplasm Metastasis; Protein Binding

Emerging evidence has indicated that deregulation of long non‐coding RNA s (lnc s) can contribute to the progression and metastasis human cancer, including hepatocellular carcinoma ( HCC ). However, roles most lnc in remain largely unknown. Here we found a noncoding termed Sch LAH (seven chromosome locus associated with ; also called BC 035072) was generally downregulated . Low expression significantly correlated shorter overall survival patients. In vitro vivo assays overexpression inhibited migration lung cells. Knockdown by si pool promoted pull‐down immunoprecipitation demonstrated physically interacted fused sarcoma FUS PCR array analysis showed RhoA Rac1 were downstream effector molecules during metastasis. rescued mRNA levels repressed These results suggest may suppress cells interacting , which indicates potential for prognosis treatment