Co‐delivery of microRNA‐21 antisense oligonucleotides and gemcitabine using nanomedicine for pancreatic cancer therapy

• DOI: 10.1111/cas.13267
• Publication Date: 2017-04-26T15:49:11Z
• Authors: Yaqing Li; Yinting Chen; Jiajia Li; Zuoquan Zhang; Chumei Huang; Guoda Lian; Kege Yang; Shaojie Chen; Ying Lin; Lingyun Wang; Kaihong Huang; Linjuan Zeng
• Keywords: Polyethylenimine; Growth inhibition

Tumor metastasis occurs naturally in pancreatic cancer, and the efficacy of chemotherapy is usually poor. Precision medicine, combining downregulation target genes with drugs, expected to improve therapeutic effects. Therefore, we developed a combined therapy micro RNA ‐21 antisense oligonucleotides ( ASO ‐miR‐21) gemcitabine (Gem) using targeted co‐delivery nanoparticle NP ) carrier investigated synergistic inhibitory effects on cancer cells growth. Polyethylene glycol–polyethylenimine–magnetic iron oxide s were used co‐deliver ‐miR‐21 Gem. An anti‐ CD 44v6 single‐chain variable fragment (scFv was coat particles obtain active delivery. Our results showed that oncogenic miR‐21 by resulted upregulation tumor‐suppressor PDCD 4 PTEN suppression epithelial–mesenchymal transition, which inhibited proliferation induced clonal formation, migration, invasion vitro . The Gem more cell apoptosis growth greater extent than single or treatment In animal tests, scFv ‐ PEG ‐polyethylenimine/ ‐magnetic /Gem accumulated at tumor site non‐targeted potent inhibition metastasis. Magnetic resonance imaging observed homing s. These imply combination gene silencing an scFv‐functionalized exerted antitumor cells, promising strategy for therapy.