Anti‐tumor effects of mAb against l‐type amino acid transporter 1 (LAT1) bound to human and monkey LAT1 with dual avidity modes

• DOI: 10.1111/cas.13908
• Publication Date: 2018-12-10T10:23:30Z
• Authors: Shiho Ueda; Hidemi Hayashi; Takako Miyamoto; Shinya Abe; Kana Hirai; Kanji Matsukura; Hideki Yagi; Yuta Hara; Kinji Yoshida; Shogo Okazaki; Masakazu Tamura; Yuki Abe; Toshinori Agatsuma; Shin‐ichiro Niwa; Kazue Masuko; Takashi Masuko
• Keywords: Internalization; HEK 293 cells; Avidity

l ‐Type amino acid transporter 1 ( LAT 1) disulfide linked to CD 98 heavy chain (hc) is highly expressed in most cancer cells, but weakly normal cells. In the present study, we developed novel anti‐ mA bs and showed internalization activity, inhibitory effects of uptake cell growth antibody‐dependent cellular cytotoxicity, as well vivo antitumor athymic mice. Furthermore, examined reactivity with Macaca fascicularis evaluate possible side‐effects antihuman clinical trials. Antihuman reacted ACHN human MK .P3 macaca kidney‐derived this was significantly decreased by si RNA s against 1. cDNA cloned from .P3, only two differences between were seen. RH 7777 rat hepatoma HEK 293 embryonic kidney cells expressing established stable transfectants, equivalently reactive transfectants or Dual (high low) avidity modes detected 1, HCT 116 colon K A values increased 98hc b, suggesting detect an epitope on 1‐ complexes surface. Based these results, may be a promising anticancer target can used preclinical studies LAT1 bs.