MicroRNA‐338‐5p reverses chemoresistance and inhibits invasion of esophageal squamous cell carcinoma cells by targeting Id‐1

• DOI: 10.1111/cas.14220
• Publication Date: 2019-10-24T05:23:43Z
• Authors: Liang Han; Di Cui; Bin Li; Wen Wen Xu; Alfred King Y. Lam; Kin Tak Chan; Yun Zhu; Nikki P.Y. Lee; Simon Y.K. Law; Xin Yuan Guan; Yan Ru Qin; Kwok Wah Chan; Stephanie Ma; Sai Wah Tsao; Annie L.M. Cheung
• Keywords: Adult; Inhibitor of Differentiation Protein 1; Male; Esophageal Neoplasms; Mice; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Animals; Humans; Neoplasm Invasiveness; Aged; 0303 health sciences; Science & Technology; chemoresistance; Oncology and carcinogenesis; Original Articles; Middle Aged; circulating miRNA; MicroRNAs; Pharmacology and pharmaceutical sciences; Drug Resistance, Neoplasm; Female; Esophageal Squamous Cell Carcinoma; Fluorouracil; Life Sciences & Biomedicine

5-Fluorouracil (5-FU) is a chemotherapeutic agent commonly used to treat esophageal squamous cell carcinoma (ESCC), but acquisition of chemoresistance frequently occurs and the underlying mechanisms are not fully understood. We found that microRNA (miR)-338-5p was underexpressed in ESCC cells with acquired 5-FU chemoresistance. Forced expression miR-338-5p these resulted downregulation Id-1, restoration both vitro vivo sensitivity treatment. The effects were abolished by reexpression Id-1. In contrast, knockdown induced resistance chemosensitive lines, Id-1 resensitized 5-FU. addition, had suppressive on migration invasion cells. Luciferase reporter assay confirmed direct interaction between 3'-UTR also significantly downregulated tumor tissue serum samples patients ESCC. Notably, low level associated poorer survival poor response 5-FU/cisplatin-based neoadjuvant chemoradiotherapy. summary, we can modulate inhibit invasion-related functions negatively regulating could be novel noninvasive prognostic predictive biomarker