Aldolase A promotes epithelial‐mesenchymal transition to increase malignant potentials of cervical adenocarcinoma

• DOI: 10.1111/cas.14524
• Publication Date: 2020-06-12T13:18:17Z
• Authors: Yuki Saito; Akira Takasawa; Kumi Takasawa; Tomoyuki Aoyama; Taishi Akimoto; Misaki Ota; Kazufumi Magara; Masaki Murata; Yoshihiko Hirohashi; Tadashi Hasegawa; Norimasa Sawada; Tsuyoshi Saito; Makoto Osanai
• Keywords: Tumor progression; Warburg Effect

Abstract Recent studies have revealed that metabolic reprogramming is closely associated with epithelial‐mesenchymal transition (EMT) during cancer progression. Aldolase A (ALDOA) a key glycolytic enzyme highly expressed in several types of cancer. In this study, we found ALDOA uterine cervical adenocarcinoma and high expression promotes EMT to increase malignant potentials, such as metastasis invasiveness, cells. human surgical specimens, was correlated lymph node metastasis, lymphovascular infiltration, short overall survival. Suppression significantly reduced cell growth, migration, invasiveness partially regulated by hypoxia‐inducible factor‐1α (HIF‐1α). Shotgun proteome analysis cell‐cell adhesion‐related proteins were increased ALDOA‐overexpressing Interestingly, overexpression caused severe morphological changes, including cuboidal‐to‐spindle shape shift microvilli formation, coincident modulation the typical EMT‐related proteins. Overexpression migration invasion vitro. Furthermore, induced HIF‐1α, suggesting positive feedback loop between HIF‐1α. conclusion, overexpressed contributes potentials tumor cells through HIF‐1α signaling. The could become therapeutic target improve prognosis malignancy.