MZT2A promotes NSCLC viability and invasion by increasing Akt phosphorylation via the MOZART2 domain

• DOI: 10.1111/cas.14900
• Publication Date: 2021-03-23T07:32:23Z
• Authors: Huanxi Wang; Xizi Jiang; Yu Cheng; Hongjiu Ren; Yujiao Hu; Yao Zhang; Hongbo Su; Zifang Zou; Qiongzi Wang; Zongang Liu; Jiameng Zhang; Xueshan Qiu
• Keywords: Lung Neoplasms; Cell Survival; Original Articles; Prognosis; Gene Expression Regulation, Neoplastic; Mice; 03 medical and health sciences; 0302 clinical medicine; Protein Domains; Antigens, Neoplasm; Carcinoma, Non-Small-Cell Lung; Cell Line, Tumor; Biomarkers, Tumor; Animals; Humans; Neoplasm Invasiveness; Phosphorylation; Microtubule-Associated Proteins; Proto-Oncogene Proteins c-akt; Signal Transduction

Abstract Mitotic spindle organizing protein 2A (MZT2A) is localized at the centrosome and regulates microtubule nucleation activity in cells. This study assessed role of MZT2A non–small‐cell lung cancer (NSCLC). Differential expression was bioinformatically using TCGA database, GEPIA Kaplan‐Meier survival data to determine association between NSCLC prognosis. Furthermore, tissue specimens were evaluated by immunohistochemistry. overexpressed or knocked down cells cDNA siRNA, respectively. The subjected various assays treated with selective Akt inhibitor LY294002 co‐transfected galectin‐3‐binding (LGALS3BP) siRNA. mRNA levels upregulated lesions MTZ2A associated poor also highly expressed compared normal bronchial promoted cell viability invasion, whereas siRNA had opposite effect on vitro. At level, induced phosphorylation, promoting proliferation invasion (but blocked these effects) through upregulation LGALS3BP via MOZART2 domain, suppressed limited vivo experiments confirmed vitro data. In conclusion, exhibits oncogenic activating NSCLC. Future studies will assess as a biomarker predict prognosis target control progression.