ELOVL5‐mediated fatty acid elongation promotes cellular proliferation and invasion in renal cell carcinoma
0301 basic medicine
03 medical and health sciences
Acetyltransferases
Fatty Acid Elongases
Fatty Acids
Humans
ORIGINAL ARTICLES
Carcinoma, Renal Cell
Proto-Oncogene Proteins c-akt
Kidney Neoplasms
Cell Proliferation
3. Good health
DOI:
10.1111/cas.15454
Publication Date:
2022-06-07T07:16:39Z
AUTHORS (14)
ABSTRACT
Renal cell carcinoma (RCC) features altered lipid metabolism and accumulated polyunsaturated fatty acids (PUFAs). Elongation of very long-chain acid (ELOVL) family enzymes catalyze elongation, ELOVL5 is indispensable for PUFAs but its role in RCC progression remains unclear. Here, we show that higher levels correlate with poor clinical prognosis. Liquid chromatography/electrospray ionization-tandem mass spectrometry analysis showed decreases end products (arachidonic eicosapentaenoic acid) under CRISPR/Cas9-mediated knockout while supplementation these partially reversed the cellular proliferation invasion effects knockout. Regarding invasion, suppressed formation droplets induced apoptosis via endoplasmic reticulum stress suppressing renal cancer vivo tumor growth. Furthermore, inhibited AKT Ser473 phosphorylation through chemokine (C-C motif) ligand-2 downregulation by AKT-mTOR-STAT3 signaling. Collectively, results suggest ELOVL5-mediated elongation promotes not only also RCC.
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CITATIONS (23)
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