ELOVL5‐mediated fatty acid elongation promotes cellular proliferation and invasion in renal cell carcinoma

0301 basic medicine 03 medical and health sciences Acetyltransferases Fatty Acid Elongases Fatty Acids Humans ORIGINAL ARTICLES Carcinoma, Renal Cell Proto-Oncogene Proteins c-akt Kidney Neoplasms Cell Proliferation 3. Good health
DOI: 10.1111/cas.15454 Publication Date: 2022-06-07T07:16:39Z
ABSTRACT
Renal cell carcinoma (RCC) features altered lipid metabolism and accumulated polyunsaturated fatty acids (PUFAs). Elongation of very long-chain acid (ELOVL) family enzymes catalyze elongation, ELOVL5 is indispensable for PUFAs but its role in RCC progression remains unclear. Here, we show that higher levels correlate with poor clinical prognosis. Liquid chromatography/electrospray ionization-tandem mass spectrometry analysis showed decreases end products (arachidonic eicosapentaenoic acid) under CRISPR/Cas9-mediated knockout while supplementation these partially reversed the cellular proliferation invasion effects knockout. Regarding invasion, suppressed formation droplets induced apoptosis via endoplasmic reticulum stress suppressing renal cancer vivo tumor growth. Furthermore, inhibited AKT Ser473 phosphorylation through chemokine (C-C motif) ligand-2 downregulation by AKT-mTOR-STAT3 signaling. Collectively, results suggest ELOVL5-mediated elongation promotes not only also RCC.
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