M6A demethylase fat mass and obesity‐associated protein regulates cisplatin resistance of gastric cancer by modulating autophagy activation through ULK1

• DOI: 10.1111/cas.15469
• Publication Date: 2022-06-22T07:55:35Z
• Authors: Yan Zhang; Ling‐xi Gao; Wen Wang; Teng Zhang; Fang‐yi Dong; Wen‐ping Ding
• Keywords: Demethylase; ULK1

Drug resistance is an important factor for treatment failure of gastric cancer. N6 -methyladenosine (m6 A) the predominant mRNA internal modification in eukaryotes. The roles m6 A drug cancer remains unclear. In present study, methylated RNA level was significantly decreased while expression demethylase fat mass and obesity-associated protein (FTO) obviously elevated cisplatin-resistant (SGC-7901/DDP) cells. Knockdown FTO reversed cisplatin SGC-7901/DDP cells both vitro vivo, which attributed to inhibition Unc-51-like kinase 1 (ULK1)-mediated autophagy. Mechanistically, ULK1 regulated FTO-m6 A-dependent YTHDF2-mediated manner. Collectively, our findings indicate that FTO/ULK1 axis exerts crucial