Single‐Nucleus RNA Sequencing and Spatial Transcriptomics for Squamous Cell Carcinoma Arising From Ovarian Mature Teratoma

• DOI: 10.1111/cas.70022
• Publication Date: 2025-02-14T04:49:34Z
• Authors: Kosuke Yoshida; Akira Yokoi; Hironori Suzuki; Satoshi Tamauchi; Masami Kitagawa; Eri Inami; Jun Nakayama; Yutaro Mori; Koji Okamoto; Yutaka Suzuki; Hiroshi Yoshida; Tomoyasu Kato; Hiroaki Kajiyama; Yusuke Yamamoto

ABSTRACT Squamous cell carcinoma arising from mature teratoma (SCC‐MT) is a rare ovarian malignancy. The detailed molecular pathology of SCC‐MT not well understood. Moreover, the prognosis patients remains poor because no standard treatment has been established. In this study, we performed single‐nucleus RNA sequencing and spatial transcriptomics using clinical samples to identify novel therapeutic candidates. snRNA‐seq revealed three epithelial clusters, which one was significantly associated with epidermis keratinocyte development. that epithelial‐mesenchymal transition inhibited, MYC E2F targets were activated in cancer spots on specimen sections. We focused KLF5, upregulated genes cells, functional analysis NOSCC‐1, line derived an SCC‐MT. KLF5 downregulation decreased proliferation increased apoptosis. Furthermore, previously identified miR‐145‐5p as downregulated miRNA demonstrated overexpression attenuated expression. conclusion, through multi‐omics analyses, unique gene expression profiles determined role for Therefore, KLF5‐related factors may be targets, further studies are needed improve diagnosis