Methyl brevifolincarboxylate, a novel influenza virus PB2 inhibitor from Canarium Album (Lour.) Raeusch

• DOI: 10.1111/cbdd.13740
• Publication Date: 2020-06-10T05:33:32Z
• Authors: Fangzhao Chen; Luoping Yang; Lingyan Zhai; Yingna Huang; Feimin Chen; Wenjun Duan; Jie Yang
• Keywords: 0303 health sciences; Influenza A Virus, H3N2 Subtype; Chick Embryo; Microbial Sensitivity Tests; RNA-Dependent RNA Polymerase; Real-Time Polymerase Chain Reaction; Antiviral Agents; Madin Darby Canine Kidney Cells; 3. Good health; Viral Proteins; 03 medical and health sciences; Dogs; HEK293 Cells; Influenza A Virus, H1N1 Subtype; Animals; Humans; Benzopyrans; Burseraceae

Abstract Methyl brevifolincarboxylate (MBC) was isolated from ethyl acetate extract of Canarium album (Lour.) Raeusch. The structure identified, and the effect on influenza A virus infection evaluated. MBC exhibited inhibitory activity against A/Puerto Rico/8/34 (H1N1) A/Aichi/2/68 (H3N2) with IC 50 values 27.16 ± 1.39 μM 33.41 2.34 μM. Mechanism studies indicated that inhibited replication by targeting PB2 cap‐binding domain. Our results demonstrated a potent inhibitor represented as new type promising lead compound for development anti‐influenza drugs natural products.