W2476 represses TXNIP transcription via dephosphorylation of FOXO1 at Ser319

• DOI: 10.1111/cbdd.13828
• Publication Date: 2021-02-10T06:28:28Z
• Authors: Li Zhong; Qing Liu; Qiaofeng Liu; Shikai Zhang; Yongbing Cao; Dehua Yang; Ming‐Wei Wang
• Keywords: Male; 0301 basic medicine; Transcription, Genetic; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Adenine; Cell Cycle Proteins; Nerve Tissue Proteins; Rats; 03 medical and health sciences; Glucose; Insulin-Secreting Cells; Animals; Phosphorylation; Rats, Wistar; Promoter Regions, Genetic; Proto-Oncogene Proteins c-akt; Cells, Cultured; Protein Binding

AbstractThioredoxin‐interacting protein (TXNIP) overexpression is implicated in the pathogenesis of type 2 diabetes. Previous studies have shown that a small molecule compound (W2476) was able to improve β‐cell dysfunction and exert therapeutic effects in diabetic mice via repression of TXNIP signaling pathway. The impact of W2476 on TXNIP transcription was thus investigated using the chromatin immunoprecipitation method. It was found that W2476 promotes competitive binding of forkhead box O1 transcription factor (FOXO1) to the carbohydrate response element (ChoRE) sequence associated with ChoRE‐binding protein (ChREBP)/Mlx interacting protein‐like(Mlx) complexes. This interaction hinders the attachment of histone acetyltransferase p300 and reduces histone H4 acetylation on the TXNIP promoter, leading to decreasing TXNIP transcription.