Serum metabolomics reveals pathways and biomarkers associated with asthma pathogenesis

• DOI: 10.1111/cea.12089
• Publication Date: 2013-01-18T08:32:46Z
• Authors: J. Jung; S.‐H. Kim; H.‐S. Lee; G. S. Choi; Y.‐S. Jung; D. H. Ryu; H.‐S. Park; G.‐S. Hwang
• Keywords: Pathogenesis; Choline

Summary Background Asthma is a chronic inflammatory disease caused by complex interactions of genetic, epigenetic, and environmental factors. For this reason, new approaches are required to clarify the pathogenesis asthma systemic review. Objective We applied 1 H‐ NMR metabolomics approach investigate altered metabolic pattern in sera from patients with sought identify mechanism underlying potential biomarkers. Method A global profile ( n = 39) controls 26) was generated using spectroscopy coupled multivariate statistical analysis. Endogenous metabolites serum were rapidly measured target‐profiling procedure. Results Multivariate analysis showed clear distinction between healthy subjects. Sera characterized increased levels methionine, glutamine, histidine decreased formate, methanol, acetate, choline, O‐phosphocholine, arginine, glucose. The detected involved hypermethylation, response hypoxia, immune reaction. Furthermore, correlated severity; particular, lipid metabolism lower forced expiratory volume s percentage (FEV %) predicted values. In addition, biomarkers strong predictive power ROC analysis, presence external validation models high accuracy (90.9% for 100% control subjects). Conclusion & Clinical Relevance These data that ‐based metabolite profiling may be useful effective diagnosis further understanding its pathogenesis.