Immunoproteomic analysis of house dust mite antigens reveals distinct classes of dominant T cell antigens according to function and serological reactivity

• DOI: 10.1111/cea.12829
• Publication Date: 2016-09-29T17:04:18Z
• Authors: C. Oseroff; L. H. Christensen; L. Westernberg; J. Pham; J. Lane; S. Paul; J. Greenbaum; T. Stranzl; G. Lund; I. Hoof; J. Holm; P. A. Würtzen; K. H. Meno; A. Frazier; V. Schulten; P. S. Andersen; B. Peters; A. Sette
• Keywords: Proteome

House dust mite (HDM) allergens are a common cause of allergy and allergic asthma. A comprehensive analysis proteins targeted by T cells, which implicated in the development regulation disease independent their antibody reactivity, is still lacking.To comprehensively analyse HDM-derived protein targets cell responses HDM-allergic individuals, investigate correlation with IgE/IgG function.Proteomic (liquid chromatography-tandem mass spectrometry) HDM extracts identified 90 distinct clusters, corresponding to 29 known 61 novel proteins. Peripheral blood mononuclear cells (PBMC) from 20 individuals were stimulated assayed set ~2500 peptides derived these clusters predicted bind most HLA class II types. 2D immunoblots made parallel elucidate IgE IgG putative function analyses performed silico according Gene Ontology annotations.Analysis reactivity revealed large number epitopes. Overall response magnitude frequency was comparable for proteins, 15 antigens (nine novel) dominating total response. Most that dominant at level also reactive, as expected, while few reactive. Among allergens, hydrolase activity detectable strongly correlated, no correlates immunogenicity 106 epitopes accounted half response, underlining heterogeneity allergens.Herein, we define both may inform future diagnostics immunotherapeutics HDM.