Glucagon‐like peptide‐1 receptor expression on human eosinophils and its regulation of eosinophil activation

• DOI: 10.1111/cea.12860
• Publication Date: 2016-12-08T06:16:42Z
• Authors: P. D. Mitchell; B. M. Salter; J. P. Oliveria; A. El‐Gammal; D. Tworek; S. G. Smith; R. Sehmi; G. M. Gauvreau; M. Butler; P. M. O'Byrne
• Keywords: Adult; Male; 0301 basic medicine; Gene Expression; Allergens; Middle Aged; Asthma; Bronchial Provocation Tests; Glucagon-Like Peptide-1 Receptor; Respiratory Function Tests; 3. Good health; Eosinophils; Immunomodulation; Young Adult; 03 medical and health sciences; Humans; Female; Methacholine Chloride

Glucagon-like peptide-1 (GLP-1) and its receptor are part of the incretin family hormones that regulate glucose metabolism. GLP-1 also has immune modulatory roles.To measure expression (GLP-1R) on eosinophils neutrophils in normal asthmatic subjects evaluate effects a analog eosinophil function.Peripheral blood samples were taken from 10 allergic subjects. GLP-1R was measured neutrophils. Subsequently, underwent allergen diluent inhalation challenges, measured. Purified eosinophils, collected mild subjects, stimulated with lipopolysaccharide (LPS) to cell activation markers CD11b CD69 cytokine (IL-4, IL-5, IL-8 IL-13) production.Glucagon-like is expressed human Eosinophil, but not neutrophil, significantly higher controls compared asthmatics. The did change either or following challenge. A decreased eosinophil-surface LPS stimulation production IL-4, IL-13, IL-5.Glucagon-like attenuates LPS-stimulated activation. agonists may have additional adjunctive indications treating persons concomitant type 2 diabetes mellitus asthma.