Subpollen particles are rich carriers of major short ragweed allergens and NADH dehydrogenases: quantitative proteomic and allergomic study

• DOI: 10.1111/cea.12874
• Publication Date: 2016-12-21T13:32:34Z
• Authors: K. Smiljanic; D. Apostolovic; S. Trifunovic; J. Ognjenovic; M. Perusko; L. Mihajlovic; L. Burazer; M. van Hage; T. Cirkovic Velic...
• Keywords: Food allergens

Short ragweed (Ambrosia artemisiifolia) allergies affect more than 36 million people annually. Ragweed pollen grains release subpollen particles (SPP) of respirable size upon hydration or a change in air electrical conditions. The aim this study was to characterize the proteomes and allergomes short SPP total protein extract (TOT), compare their effects with those standard aqueous (APE) using sera from pollen-sensitized patients.Quantitative 2D gel-based shotgun proteomics, 1D immunoblotting, quantitative ELISA were applied. Novel extraction preparation protocols enabled appropriate sample further downstream analysis by proteomics.The fraction contained highest proportion (94%) allergome, largest quantities minor Amb 4 major 1 allergens, as unique, NADH dehydrogenases. APE richest 6, 5 3, TOT 8 allergens (89% 83% respectively). Allergenic potency correlated well among three fractions tested, immunoblots demonstrating slight predominance IgE reactivity compared APE. However, strongest binding noted against New allergenic candidates, phosphoglycerate mutase phosphoglucomutase, identified all fractions. Enolase, UTP-glucose-1-phosphate uridylyltransferase polygalacturonase observed novel pollen, previously described.We demonstrated that complete (Amb 11) almost 4, 5, 9) allergen repertoire oxidases are present SPP, highlighting an important role for allergic sensitization ragweed.