Therapeutic administration of bone marrow‐derived mesenchymal stromal cells reduces airway inflammation without up‐regulating Tregs in experimental asthma

• DOI: 10.1111/cea.13048
• Publication Date: 2017-10-25T17:20:46Z
• Authors: J. Z. Kitoko; L. L. de Castro; A. P. Nascimento; S. C. Abreu; F. F. Cruz; A. C. Arantes; D. G. Xisto; M. A. Martins; M. M. Morales; P. R. M. Rocco; P. C. Olsen
• Keywords: 0301 basic medicine; Biopsy; Pyroglyphidae; Mesenchymal Stem Cells; Mice, Transgenic; Cell Communication; Allergens; Lymphocyte Activation; Mesenchymal Stem Cell Transplantation; T-Lymphocytes, Regulatory; Asthma; Coculture Techniques; Respiratory Function Tests; 3. Good health; Immunomodulation; Disease Models, Animal; Mice; 03 medical and health sciences; Animals

Summary Background Prophylactic administration of mesenchymal stromal cells ( MSC s) derived from adipose AD ‐ ) and bone marrow tissue BM in ovalbumin‐induced asthma hinders inflammation a Treg‐dependent manner. It is uncertain whether s act through Tregs when already established induced by clinically relevant allergen. Objective Evaluate the effect therapeutic on Treg house dust mite HDM )‐induced asthma. Methods were administered intratracheally to C57 BL /6 mice 1 day after last challenge. Lung function, remodelling parenchymal assayed 3 or 7 days treatment, invasive plethysmography histology, respectively. Bronchoalveolar lavage fluid BALF mediastinal lymph nodes mLN assessed regarding inflammatory profile flow cytometry, ELISA qRT‐PCR. studied their potential induce primed unprimed lymphocytes vitro. Results s, but not reduced lung influx eosinophils B increased IL ‐10 levels ‐challenged mice. Neither nor inflammation, airway hyperresponsiveness mucus hypersecretion. did up‐regulate cell counts within airways decreased pro‐inflammatory alveolar macrophages. Co‐culture with allergen‐stimulated cell‐to‐cell contact‐independent manner, although co‐culture both up‐regulated counts. Conclusions therapeutically exert anti‐inflammatory effects mice, do ameliorate function remodelling. Although pre‐treatment can increase numbers, it highly unlikely that will expansion are allergenically an inflammation.