An integrated framework using high‐dimensional mass cytometry and fluorescent flow cytometry identifies discrete B cell subsets in patients with red meat allergy

• DOI: 10.1111/cea.13322
• Publication Date: 2018-12-02T11:24:06Z
• Authors: Kelly M. Cox; Scott P. Commins; Brian J. Capaldo; Lisa J. Workman; Thomas A. E. Plat...; El‐ad D. Amir; Josephine A. Lann...; Alexander J. Schu...; Loren D. Erickson
• Keywords: ELISPOT; Immunoglobulin D; Cytometry; Mass cytometry

Summary Background B cells play a critical role in the development and maintenance of food allergy by producing allergen‐specific IgE. Despite importance IgE‐mediated allergy, identity sIgE ‐producing human how IgE is regulated are poorly understood. Objective To identify immunophenotypes circulating associated with production galactose‐alpha‐1,3‐galactose‐specific patients red meat allergy. Methods PBMC samples obtained from 19 adults physician‐diagnosed 20 non‐meat allergic healthy controls were assessed mass cytometry along bioinformatics analysis pipeline to discrete cell phenotypes that serum . Fluorescent flow was then applied sort purify subsets, functionally evaluated on an individual level for ELISPOT. Results Discrete abundant subjects compared found peripheral blood do not share typical characteristics classical isotype‐switched memory express high levels CD 27. These subsets shared higher IgD lower IgM expression coupled CXCR 4, CCR 6 25 expression. In vitro polyclonal stimulation purified demonstrated these enriched induced secrete Conclusions Clinical Relevance Circulating display increased abundance subjects. This observation, capacity produce following activation, implicates novel promoting