Recombinant T‐cell epitope conjugation: A new approach for Dermatophagoides hypoallergen design

• DOI: 10.1111/cea.14238
• Publication Date: 2022-09-30T05:50:05Z
• Authors: Antônio Márcio Sa...; Eduardo Santos da...; Elisânia Fontes S...; Emília Maria Mede...; Leonardo Freire S...; Raphael Chagas Silva; Vitor dos Santos ...; Deise Malta Carneiro; Fatima Ferreira; Alain Jacquet; Luis Gustavo Carv...; Neuza Maria Alcan...; Carina Silva Pinh...
• Keywords: Hypoallergenic

Allergen-specific immunotherapy (AIT) is the only clinical approach that can potentially cure some allergic diseases by inducing immunological tolerance. Dermatophagoides pteronyssinus considered as most important source of mite allergens worldwide, with high sensitization rates for major Der p 1, 2 and 23. The aim this work to generate a hypoallergenic hybrid molecule containing T-cell epitopes from these three allergens.The protein termed 2231 was purified affinity chromatography. human IgE reactivity verified comparing those parental allergens. also characterized immunologically through an in vivo mice model.The rDer stimulated peripheral blood mononuclear cells (PBMCs) isolated patients higher levels IL- 2, IL-10, IL-15 IFN-γ, well lower IL-4, IL-5, IL-13, TNF-α GM-CSF. use molecules therapeutic model D. led reduction production eosinophilic peroxidase activity airways. We found increased IgG antibodies blocked binding serum patients. Furthermore, stimulation splenocytes treated induced IL-10 IFN-γ decreased secretion IL-4 when compared extract.rDer has potential be used AIT co-sensitized allergens, once it able reduce production, allergen-specific blocking antibodies, restoring balancing Th1/Th2 immune responses, regulatory T-cells.