Ex-vivotolerogenic F4/80+ antigen-presenting cells (APC) induce efferent CD8+ regulatory T cell-dependent suppression of experimental autoimmune uveitis

• DOI: 10.1111/cei.12243
• Publication Date: 2013-11-25T11:08:35Z
• Authors: S-M Hsu; R Mathew; A W Taylor; J Stein-Streilein
• Keywords: Peripheral tolerance; Ex vivo

Summary It is known that inoculation of antigen into the anterior chamber (a.c.) a mouse eye induces a.c.-associated immune deviation (ACAID), which mediated in part by antigen-specific local and peripheral tolerance to inciting antigen. ACAID can also be induced vivo intravenous (i.v.) ex-vivo-generated tolerogenic antigen-presenting cells (TolAPC). The purpose this study was test if in-vitro-generated retinal antigen-pulsed TolAPC suppressed established experimental autoimmune uveitis (EAU). Retinal were injected i.v. mice 7 days post-induction EAU. We observed incidence severity clinical expression EAU reduced associated inflammatory cytokines. Moreover, extract whole retina efficiently replaced interphotoreceptor retinoid-binding protein (IRBP) preparation used induce mice. Finally, suppression could transferred new set with CD8+ but not CD4+regulatory T (Treg). ongoing inducing Treg that, turn, effector activity IRBP-specific altered symptoms inflammation eye. ability use for raises possibility produce autologous then as therapy human uveitis.