A single administration of human adipose tissue-derived mesenchymal stromal cells (MSC) induces durable and sustained long-term regulation of inflammatory response in experimental colitis

• DOI: 10.1111/cei.13262
• Publication Date: 2019-01-21T08:55:40Z
• Authors: V B Freitas Alves; B Coutinho de Sousa; M Thaís Costa Fon...; H Ogata; C Caliári-Oliveira; J Navarro Ueda Ya...; V Rodrigues Júnior; J E Lazo Chica; J Santana da Silva; K C Ribeiro Malme...; L Pernomian; C Ribeiro Cardoso
• Keywords: Mesenteric lymph nodes

Summary Current therapies for inflammatory bowel diseases (IBD) are aimed at controlling the exacerbated response in gut, but no treatment is fully effective many refractory patients. Mesenchymal stromal cells (MSC) multi-potent with regulatory immunosuppressive activity that may control diseases. In this study, we investigated short- and especially long-term protective effects of MSC on experimental colitis. We show elicited protection to acute intestinal inflammation gain weight, improvement clinical disease score expressive reduction mortality rate treated mice. changed population neutrophils, eosinophils augmented frequency CD4 T lymphocytes gut-draining lymph nodes, together reduced accumulation these colon intraepithelial compartment. Interestingly, there were increased levels programmed death 1 (PD-1) glucocorticoid-induced tumour necrosis factor receptor family-related (GITR) spleen mice received treatment, which also presented a reversal pattern immune diminished inflammatory, helper type (Th1) Th17 profile, contrast Th2 responses. Most strikingly, balanced by single administration during colitis persisted long-term, restored goblet cells, maintenance elevated gut interleukin (IL)-4, besides CD4+CD25+PD-1+ mesenteric nodes (MLN) day 60. Taken together, our findings provided significant contribution translational immunology pointing human adipose tissue-derived as novel therapeutic approach beneficial