Presence of intragraft B cells during acute renal allograft rejection is accompanied by changes in peripheral blood B cell subsets

• DOI: 10.1111/cei.13269
• Publication Date: 2019-02-03T11:08:28Z
• Authors: S Heidt; M Vergunst; J D H Anholts; G M J S Swings; E M J Gielis; K E Groeneweg; M J Witkamp; J W de Fijter; M E J Reinders; D L Roelen; M Eikmans; F H J Claas
• Keywords: CXCL13; Naive B cell; CXCR5

Summary B cells have various functions, besides being plasma cell precursors. We determined the presence of intragraft at time acute rejection (AR) and looked for correlates involvement in peripheral blood. Renal biopsies AR or stable graft function were analysed cell-related gene expression, as well C4d staining. Peripheral blood subset distribution was time-points patients with controls, alongside serum human leucocyte antigen (HLA) antibodies. accompanied by CD20+ cells, elevated CD20 (MS4A1) CD19 expression compared to controls. infiltrates proportional T chemokine pair C-X-C motif ligand 13 (CXCL13)–C-X-C receptor 5 (CXCR5) activating factor (BAFF). memory decreased naive increased AR, contrast While 22% 5% controls showed de-novo donor-specific antibodies (DSA), all C4d-negative. These results suggest a role infiltrating cells. hypothesize that shift composition is related infiltration AR.