Altered inflammasome activation in neonatal encephalopathy persists in childhood

• DOI: 10.1111/cei.13598
• Publication Date: 2021-03-26T08:00:41Z
• Authors: L A Kelly; M I O'Dea; Z Zareen; A M Melo; E McKenna; T Strickland; V McEneaney; V Donoghue; G Boylan; D Sweetman; J Butler; C Vavasseur; J Miletin; A F El-Khuffash; L A J O'Neill; J J O'Leary; E J Molloy
• Keywords: Neonatal Encephalopathy

Neonatal encephalopathy (NE) is characterized by altered neurological function in term infants and inflammation plays an important pathophysiological role. Inflammatory cytokines interleukin (IL)-1β, IL-1ra IL-18 are activated the nucleotide-binding oligomerization domain (NOD)-, leucine-rich repeat (LRR)- NOD-like receptor protein 3 (NLRP3) inflammasome; furthermore, we aimed to examine role of inflammasome multiprotein complex involved proinflammatory responses from newborn period childhood NE. Cytokine concentrations were measured multiplex enzyme-linked immunosorbent assay (ELISA) neonates children with NE absence or presence lipopolysaccharide (LPS) endotoxin. We then investigated expression NLRP3 genes, NLRP3, IL-1β ASC polymerase chain reaction (PCR). Serum samples 40 patients at days 1 first week life 37 age 4-7 years analysed. An increase serum on was observed compared neonatal controls. decreased normal levels school age, whereas even higher controls life. Percentage LPS response newborns school-age gene up-regulated remained Increased activation day persists childhood, may window for therapeutic intervention.