Vitamin D status is independently associated with plasma glutathione and cysteine thiol/disulphide redox status in adults

• DOI: 10.1111/cen.12449
• Publication Date: 2014-03-13T17:28:45Z
• Authors: Jessica A. Alvarez; Ritam Chowdhury; Dean P. Jones; Greg S. Martin; Kenneth L. Brigham; José N. Binongo; Thomas R. Ziegler; Vin Tangpricha
• Keywords: Thiol; Plasma levels

Summary Objective Redox status and inflammation are important in the pathophysiology of numerous chronic diseases. Epidemiological studies have linked vitamin D to a number We aimed examine relationships between serum 25‐hydroxyvitamin [25( OH )D] circulating thiol/disulphide redox biomarkers inflammation. Design This was cross‐sectional study N = 693 adults (449 females, 244 males) an apparently healthy, working cohort Atlanta, GA . Plasma glutathione ( GSH ), cysteine (Cys) their associated disulphides were determined with high‐performance liquid chromatography, potentials (E h GSSG E Cy SS ) calculated using Nernst equation. Serum inflammatory markers included interleukin‐6 IL ‐6), interleukin‐8 ‐8) tumour necrosis factor‐α, assayed on multiplex platform, C‐reactive protein CRP commercially. Relationships assessed multiple linear regression analyses. Results 25( )D positively plasma (β ± SE : 0·002 0·0004) negatively −0·06 0·01) Cys −0·01 0·003) P < 0·001 for all); statistical significance remained after adjusting age, gender, race, percentage body fat traditional cardiovascular risk factors 0·01–0·02). The inverse relationship confounded by fat, full adjustment covariates attenuated other nonstatistical significance. Conclusions concentrations independently major systems, suggesting that may be involved redox‐mediated pathophysiology.