Cytoplasmic replication ofStaphylococcus aureusupon phagosomal escape triggered by phenol-soluble modulin α

• DOI: 10.1111/cmi.12233
• Publication Date: 2013-10-25T04:44:47Z
• Authors: Magdalena Grosz; Julia Kolter; Kerstin Paprotka; Ann-Cathrin Winkler; Daniel Schäfer; Som Subra Chatterjee; Tobias Geiger; Christiane Wolz; Knut Ohlsen; Michael Otto; Thomas Rudel; Bhanu Sinha; Martin Fraunholz
• Keywords: Intracellular parasite; Phagolysosome

Staphylococcus aureus is a Gram-positive human pathogen that readily internalized by professional phagocytes such as macrophages and neutrophils but also non-professional epithelial or endothelial cells. Intracellular bacteria have been proposed to play role in evasion of the innate immune system may lead dissemination within migrating phagocytes. Further, S. efficiently lyses host cells with battery cytolytic toxins. Recently, phenol-soluble modulins (PSM) identified comprise genus-specific family peptides. Of these PSMα peptides implicated killing polymorphonuclear leucocytes after phagocytosis. We questioned if were active destroying endosomal membranes avoid lysosomal monitored integrity infected cell endosomes measuring acidity intracellular bacterial microenvironment via flow cytometry reporter recruitment technique. Isogenic mutants methicillin-resistant (MRSA) strains USA300 LAC, USA400 MW2 well strongly methicillin-sensitive strain 6850 compared their respective wild type strains. In all three genetic backgrounds, unable escape from phagosomes (293, HeLa, EAhy.926) (THP-1), whereas PSMβ δ-toxin β-toxin, phosphatidyl inositol-dependent phospholipase C Panton Valentine leucotoxin escaped efficiencies parental replicated intracellularly only presence functional operon thereby illustrating grow cytoplasm upon phagosomal escape.