Klebsiella pneumoniaesurvives within macrophages by avoiding delivery to lysosomes

• DOI: 10.1111/cmi.12466
• Publication Date: 2015-06-05T03:00:00Z
• Authors: Victoria Cano; Catalina March; Jose Luis Insua; Nacho Aguiló; Enrique Llobet; David Moranta; Verónica Regueiro; Gerard P. Brennan; Maria Isabel Mill...; Carlos Martín; Junkal Garmendia; José A. Bengoechea
• Keywords: 0303 health sciences; Microbial Viability; Macrophages; 610; 612; 3. Good health; Klebsiella pneumoniae; Mice; 03 medical and health sciences; Host-Pathogen Interactions; Vacuoles; Animals; Humans; Lysosomes; Cells, Cultured

Klebsiella pneumoniae is an important cause of community-acquired and nosocomial pneumonia. Evidence indicates that might be able to persist intracellularly within a vacuolar compartment. This study was designed investigate the interaction between macrophages. Engulfment K. dependent on host cytoskeleton, cell plasma membrane lipid rafts activation phosphoinositide 3-kinase (PI3K). Microscopy studies revealed resides compartment, Klebsiella-containing vacuole (KCV), which traffics vacuoles associated with endocytic pathway. In contrast UV-killed bacteria, majority live bacteria did not co-localize markers lysosomal Our data suggest triggers programmed death in macrophages displaying features apoptosis. efforts identify mechanism(s) whereby prevents fusion lysosomes KCV uncovered central role PI3K-Akt-Rab14 axis control phagosome maturation. capsule dispensable for intracellular survival if were opsonized. Furthermore, environment found by triggered down-regulation expression cps. Altogether, this proves evidence survives killing manipulating maturation may contribute pathogenesis.