Candida albicansis able to use M cells as a portal of entry across the intestinal barrierin vitro

• DOI: 10.1111/cmi.12495
• Publication Date: 2015-08-04T18:51:47Z
• Authors: Sandrine Albac; Antonin Schmitz; Carolina Lopez-Al...; Christophe d'Enfert; Marc Sautour; Amandine Ducreux; Catherine Labruèr...; Michael Laue; Gudrun Holland; Alain Bonnin; Frederic Dalle
• Keywords: Microfold cell; Enterocyte; Transcytosis; Internalization

Candida albicans is the most frequent yeast responsible for systemic infections in humans. These mainly originate from gastrointestinal tract where C. can invade gut epithelial barrier to gain access bloodstream. Along gut, pathogens use Microfold (M) cells as a portal of entry cross barrier. M are specialized located follicule-associated epithelium Peyer patches. In this study, we used scanning electron and fluorescence microscopy, adhesion invasion assays fungal mutants investigate interactions with obtained an established vitro model whereby enterocyte-like Caco-2 co-cultured Raji B cell line undergo phenotypic switch morphologically functionally resembling cells. Our data demonstrate that co-localizes invades preferentially cells, providing evidence fungus into intestinal addition active penetration, F-actin dependent endocytosis contributes internalization through mechanism involving hypha-associated invasins including Ssa1 Als3.