Development of an in vitro colonization model to investigateStaphylococcus aureusinteractions with airway epithelia

• DOI: 10.1111/cmi.12543
• Publication Date: 2015-11-14T00:28:52Z
• Authors: Megan R. Kiedrowski; Alexandra E. Paharik; Laynez W. Ackermann; Annie U. Shelton; Sachinkumar B. Singh; Timothy D. Starner; Alexander R. Hors...
• Keywords: 0301 basic medicine; Staphylococcus aureus; 0303 health sciences; Virulence; Bacterial Toxins; Respiratory System; Epithelial Cells; Staphylococcal Infections; Bacterial Adhesion; Coculture Techniques; 3. Good health; Hemolysin Proteins; 03 medical and health sciences; Mutation; Humans

Staphylococcus aureus is a bacterial pathogen responsible for wide range of diseases and also human commensal colonizing the upper respiratory tract. Strains belonging to clonal complex group CC30 are associated with colonization, although colonization state itself not clearly defined. In this work, we developed co-culture model S. apical surface polarized airway epithelial cells. The grown at air–liquid interface allow an in-depth evaluation simulated state. Exposure wild-type, bacteria or conditioned media killed cells within 1 day, while mutant strains lacking alpha-toxin (hla) persisted on viable least 2 days. Recent isolates natural hla mutants, observed that these displayed reduced toxicity toward Quantitative real-time polymerase chain reaction known virulence factors showed expression profile in correlates results from previous studies. Microarray analysis indicated significant shifts physiology lipid amino acid metabolism. development vitro will enable further study specific interactions host epithelia.