α-Hemolysin enhancesStaphylococcus aureusinternalization and survival within mast cells by modulating the expression of β1 integrin

• DOI: 10.1111/cmi.12550
• Publication Date: 2015-11-23T21:29:00Z
• Authors: Oliver Goldmann; Lorena Tuchscherr; Manfred Rohde; Eva Medina
• Keywords: Internalization; ADAM10

Mast cells (MCs) are important sentinels of the host defence against invading pathogens. We previously reported that Staphylococcus aureus evaded extracellular antimicrobial activities MCs by promoting its internalization within these via β1 integrins. Here, we investigated molecular mechanisms governing this process. found S. responded to mediators released up-regulating expression α-hemolysin (Hla), fibronectin-binding protein A and several regulatory systems. also induced up-regulation integrin on effect was mediated Hla-ADAM10 (a disintegrin metalloproteinase 10) interaction. Thus, deletion Hla or inhibition interaction significantly impaired MCs. Furthermore, purified but not inactive HlaH35L in a dose-dependent manner. Our data support model which counter-reacts microbicidal increasing proteins inducing Hla-ADAM10-mediated The bacterial proteins, concomitantly with increased receptor MCs, resulted enhanced through binding fibronectin.