Proteomics analysis ofMedicago truncatularesponse to infection by the phytopathogenic bacteriumRalstonia solanacearumpoints to jasmonate and salicylate defence pathways

• DOI: 10.1111/cmi.12796
• Publication Date: 2017-10-30T23:26:39Z
• Authors: Ahad Yamchi; Cécile Ben; Michel Rossignol; Sayed Reza Zareie; Aghafakhr Mirlohi; Badraldin Ebrahim...; Carole Pichereaux; Ahmad Sarrafi; Martina Rickauer; Laurent Gentzbittel
• Keywords: Medicago truncatula; Ralstonia solanacearum; Methyl jasmonate; Jasmonate

The infection of the model legume Medicago truncatula with Ralstonia solanacearum GMI1000 gives rise to bacterial wilt disease via colonisation roots. root and leaf responses early (1 3 days post infection) were characterised investigate molecular mechanisms plant resistance or susceptibility. A proteomics approach based on pools susceptible resistant recombinant inbred lines was used specifically target for tolerance. Differential abundances evidenced proteins involved in defence (e.g., PR5, PR10, Kunitz protease inhibitors) signalling pathways (such as cyclophilin). R. inoculation modifies expression levels those genes, either both genotypes (AOS1, LOX4, proteinase line (PR proteins). Exogenous application salicylic acid (SA) enhanced tolerance bacteria, whereas methyl jasmonate (MeJA) short-term then promoted ecotype, suggesting that they may mediate responses. Conversely, proteomics-identified genes also shown be SA MeJA responsive. This is first description differential response M. truncatula. Our results suggest basal activated at 1 dpi, together JA pathway. Specific three up-regulation SA-dependent PR proteins.