Discordant susceptibility of inbred C57BL/6 versus outbred CD1 mice to experimental fungal sepsis

• DOI: 10.1111/cmi.12995
• Publication Date: 2018-12-21T23:22:58Z
• Authors: Esther Carreras; María Velasco de ...; Marc Orta‐Mascaró; Inês T. Simões; Cristina Català; Oscar Zaragoza; Francisco Lozano
• Keywords: Zymosan; Lipoteichoic acid; Splenocyte

Individual susceptibility differences to fungal infection following invasive and/or immunosuppressive medical interventions are an important clinical issue. In order explore immune response-related factors that may be linked susceptibility, we have compared the response of inbred C57BL/6J and outbred CD1 mouse strains different experimental models sepsis. The challenge animals with zymosan-induced generalised inflammation model revealed poorer survival rates in C57BL/6J, consistent lower Th1 cytokine interferon (IFN)-γ serum levels, mice. Likewise, ex vivo exposure splenocytes zymosan but also bacterial lipopolisaccharide or lipoteichoic acid, resulted IFN-γ secretion could reverted by rescue infusion relative low doses (0.2 μg/kg) either alone combination ß-glucan-binding CD5 protein (0.7 mg/kg) leading improved post survival. Similarly, systemic Candida albicans (2.86 × 104 CFU/gr) were ameliorated low-dose not Our results highlight importance strain choice provide a rationale for more specific antifungal immunotherapy designs.