Virulence and pathogenicity of aCandida albicansmutant with reduced filamentation

• DOI: 10.1111/cmi.13103
• Publication Date: 2019-08-19T12:20:27Z
• Authors: Doureradjou Peroumal; Kodavati Manohar; Shraddheya Kumar ...; Premlata Kumari; Satya Ranjan Sahu; Narottam Acharya
• Keywords: Filamentation

Deletion of DNA polymerase eta (Rad30/Polη) in pathogenic yeast Candida albicans is known to reduce filamentation induced by serum, ultraviolet, and cisplatin. Because nonfilamentous C. widely accepted as avirulent form, here we explored the virulence pathogenicity a rad30Δ strain cell-based animal systems. Flow cytometry cocultured fungal differentiated macrophage cells revealed that comparatively higher percentage macrophages was associated with wild-type than cells. In contrast, number Polη-deficient adhered per membrane. Imaging flow showed developed hyphae after phagocytosis caused necrotic death evade their clearance. Conversely, phagosomes kill estimated increased metacaspase activity albicans. Despite morphological differences, both rad30∆ were virulent varying degree mice models. Notably, Th1 immunity less susceptible systemic infection Th2 type. Thus, our study clearly suggests modes interaction morphologically different strains host immune are diverged, genetic background several other attributing factors fungus could additionally determine virulence.