LrrkA, a kinase with leucine‐rich repeats, links folate sensing with Kil2 activity and intracellular killing

• DOI: 10.1111/cmi.13129
• Publication Date: 2019-10-25T19:53:20Z
• Authors: Romain Bodinier; Jade Leiba; Ayman Sabra; Tania N. Jauslin; Otmane Lamrabet; Cyril Guilhen; Anna Marchetti; Yumi Iwade; Takefumi Kawata; Wanessa C. Lima; Pierre Cosson
• Keywords: Intracellular parasite

Phagocytic cells ingest bacteria by phagocytosis and kill them efficiently inside phagolysosomes. The molecular mechanisms involved in intracellular killing their regulation are complex still incompletely understood. Dictyostelium discoideum has been used as a model to discover study new gene products of ingested bacteria. In this study, we performed random mutagenesis isolated mutant defective for growth on This is characterized the genetic inactivation lrrkA gene, which encodes protein with kinase domain leucine-rich repeats. LrrkA knockout (KO) Klebsiella pneumoniae inefficiently. defect not additive observed kil2 KO cells, suggesting that function Kil2 partially controlled LrrkA. Indeed, exhibit phenotype similar cells: Intraphagosomal proteolysis inefficient, both intraphagosomal restored upon exogenous supplementation magnesium ions. Bacterially secreted folate stimulates but stimulation lost kil2, lrrkA, or far1. Together, these results indicate involves Far1 (the cell surface receptor folate), LrrkA, Kil2. first identification signalling pathway regulating bacterial cells.