Exosomes containing miR‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury

• DOI: 10.1111/cns.13612
• Publication Date: 2021-02-06T12:01:19Z
• Authors: He Li; Yin Luo; Peng Liu; Pei Liu; Weilong Hua; Yongxin Zhang; Lei Zhang; Zifu Li; Pengfei Xing; Yongwei Zhang; Bo Hong; Pengfei Yang; Jianmin Liu
• Keywords: Male; 0303 health sciences; Behavior, Animal; Infarction, Middle Cerebral Artery; Original Articles; Cerebral Infarction; Exosomes; Neuroprotection; Mice, Inbred C57BL; Mice; MicroRNAs; 03 medical and health sciences; Brain Injuries; Cell Line, Tumor; Reperfusion Injury; Animals; Ischemic Preconditioning; Reactive Oxygen Species

Abstract Aim To study the role of exosomes in protective effect cerebral ischemic preconditioning (cerebral‐IPC) against I/R injury. Method Mouse models cerebral‐IPC and MCAO/R were established as described previously, their behavioral, pathological, proteomic changes analyzed. Neuro‐2a subjected to OGD/R treated with isolated from plasma sham‐operated mice. The differentially expressed miRNAs between derived (S‐exosomes) preconditioned (IPC‐exosomes) mice identified through miRNA array, targets database search. control cells IPC‐exosomes, mimic or target protein inhibitor, viability, oxidative, stress apoptosis rates measured. activated pathways by analyzing levels relevant proteins. Results Cerebral‐IPC mitigated injury following ischemia reperfusion, increased number exosomes. IPC‐exosomes survival after OGD/R. miR‐451a targeting Rac1 was upregulated relative S‐exosomes. inhibitor NSC23766 reversed OGD/R‐mediated activation its downstream pathways. Conclusion ameliorated inducing release containing miR‐451a.