Exploring the efficacy of tumor electric field therapy against glioblastoma: An in vivo and in vitro study

• DOI: 10.1111/cns.13750
• Publication Date: 2021-10-28T16:26:27Z
• Authors: Hao Wu; Lin Yang; Hanjie Liu; Dan Zhou; Dikang Chen; Xiaoque Zheng; Hui Yang; Chong Li; Jiusheng Chang; Anhua Wu; Zhifei Wang; Nianjun Ren; Shengqing Lv; Yuyang Liu; Muyuan Jia; Jian Lu; Hongyu Liu; Guochen Sun; Zhixiong Liu; Jialin Liu; Ling Chen
• Keywords: U87; Brain tumor

Tumor electric fields therapy (TTFields) is emerging as a novel anti-cancer physiotherapy. Despite recent breakthroughs of TTFields in glioma treatment, the average survival time for glioblastoma patients with <2 years, even when used conjugation traditional therapies. To optimize TTFields-afforded efficacy against glioblastoma, we investigated cancer cell-killing effects various paradigms using vitro and vivo models glioblastoma.For studies, U251 cell line or primary cultures prepared from 20 were treated tumor field treatment (TEFT) system. Cell number, volume, proliferation measured after TEFT at different frequencies (100, 150, 180, 200, 220 kHz), durations (24, 48, 72 h), strengths (1.0, 1.5, 2.2V/cm), output modes (fixed random sequence output). A transwell system was to evaluate influence on invasiveness cells. For therapeutic effect safety profiles glioblastoma-transplanted rats assessed by calculating size evaluating peripheral immunobiological blood parameters, respectively.In settings, robustly effective suppressing both cultures. The anti-proliferation frequency- "dose" (field strength duration)-dependent, contingent mode, mode (TEFT-R) being more than fixed (TEFT-F). Genetic tests performed 11 cultures, 6 genetic traits identified them. However, exhibited comparable all regardless their traits. also inhibited cells experiments. In rat model brain transplantation, TEFT-F TEFT-R frequency 200 kHz 2.2V/cm 14 days significantly reduced volume 42.63% (TEFT-F vs. control, p = 0.0002) 63.60% (TEFT-R < 0.0001), prolonged animal 30.15% 0.0415) 69.85% 0.0064), respectively. tumor-bearing appeared be well tolerable therapies, showing only moderate increases levels creatine red Adverse skin reactions common TEFT-treated rats; however, curable local treatment.Tumor optimal frequency, strength, markedly inhibits viability, proliferation, independent Moreover, confers considerable vivo. Thus, are promising physiotherapy warrants further investigation.