Effects of α5 GABAA receptor modulation on social interaction, memory, and neuroinflammation in a mouse model of Alzheimer's disease

• DOI: 10.1111/cns.13914
• Publication Date: 2022-07-13T11:44:42Z
• Authors: Jovana Aranđelović; Anja Santrač; Bojan Batinić; Lidija Todorović; Vladimir Stevanović; Veera Venkata Nag...; Dishary Sharmin; Farjana Rashid; Boban Stanojević; James M. Cook; Miroslav M. Savić
• Keywords: cognition; Male; 570; Social Interaction; Mice, Transgenic; neuroinflammation; Mice; 03 medical and health sciences; Alzheimer Disease; Memory; 616; Animals; gamma-Aminobutyric Acid; 0303 health sciences; Amyloid beta-Peptides; Interleukin-6; Tumor Necrosis Factor-alpha; Original Articles; Alzheimer's disease; Receptors, GABA-A; 5xFAD; α5 GABAA receptor modulation; 3. Good health; Disease Models, Animal; Neuroinflammatory Diseases; Solvents; Female

AbstractAimsGABAergic modulation involved in cognitive processing appears to be substantially changed in Alzheimer's disease (AD). In a widely used 5xFAD model of AD, we aimed to assess if negative and positive allosteric modulators of α5 GABAA receptors (NAM and PAM, respectively) would affect social interaction, social, object and spatial memory, and neuroinflammation.MethodsAfter 10‐day treatment with PAM, NAM, or solvent, 6‐month‐old transgenic and non‐transgenic 5xFAD mice underwent testing in a behavioral battery. Gene expressions of IL‐1β, IL‐6, TNF‐α, GFAP, and IBA‐1 were determined in hippocampus and prefrontal cortex by qPCR.ResultsPAM treatment impaired spatial learning in transgenic females compared to solvent‐treated transgenic females, and social recognition in transgenic and non‐transgenic males. NAM treatment declined social interaction in transgenic and non‐transgenic males, while had beneficial effect on cognitive flexibility in non‐transgenic males compared to solvent‐treated non‐transgenic males. Transgenic animals have not fully displayed cognitive symptoms, but neuroinflammation was confirmed. NAM reduced proinflammatory gene expressions in transgenic females and astrogliosis in transgenic males compared to pathological controls.ConclusionPAM and NAM failed to exert favorable behavioral effects in transgenic animals. Suppression of neuroinflammation obtained with NAM calls for more studies with GABAergic ligands in amyloid beta‐ and/or tau‐dependent models with prominent neuroinflammation.