A decrease in NR2B expression mediated by DNA hypermethylation induces perioperative neurocognitive disorder in aged mice

• DOI: 10.1111/cns.14097
• Publication Date: 2023-01-25T05:09:45Z
• Authors: Feifei Xu; Peilin Cong; Bingqian Zhang; Hailong Dong; Wenqiang Zuo; Tingmei Wu; Li Tian; Lize Xiong
• Keywords: 0301 basic medicine; Laparotomy; Original Articles; DNA; DNA Methylation; Hippocampus; Receptors, N-Methyl-D-Aspartate; Mice, Inbred C57BL; Mice; 03 medical and health sciences; 0302 clinical medicine; Animals; Learning; Cognition Disorders

Abstract Aims This study was designed to investigate the role of NR2B and contribution DNA methylation expression in pathogenesis PND. Methods Eighteen‐month‐old C57BL/6J mice were subjected experimental laparotomy under 1.4% isoflurane anesthesia. Hippocampus‐dependent learning memory evaluated by using Barnes maze contextual fear conditioning tests. The protein mRNA levels western blotting qRT–PCR respectively, gene examined targeted bisulfite sequencing. Long‐term synaptic plasticity (LTP) measured electrophysiology. Results Mice that underwent exhibited hippocampus‐dependent cognitive deficits accompanied decreased expressions LTP deficiency. overexpression dorsal hippocampus could improve laparotomy. In particular, induced attributed hypermethylation. Preoperative administration S‐adenosylmethionine (SAM) hypomethylate gene, upregulate LTP, exerting a dose‐dependent therapeutic effect against Moreover, inhibiting abrogated benefits SAM pretreatment. Conclusions Laparotomy cause decline hypermethylating allowing us understand PND an epigenetic landscape.