Circular RNA DOCK1 promotes bladder carcinoma progression via modulating circDOCK1/hsa‐miR‐132‐3p/Sox5 signalling pathway

• DOI: 10.1111/cpr.12614
• Publication Date: 2019-04-15T03:41:49Z
• Authors: Peihua Liu; Xiaozhou Li; Xi Guo; Jinbo Chen; Chao Li; Minfeng Chen; Longfei Liu; Xiangyang Zhang; Xiongbing Zu
• Keywords: 0301 basic medicine; Mice, Inbred BALB C; Cell Survival; Urinary Bladder; Mice, Nude; Original Articles; RNA, Circular; Up-Regulation; 3. Good health; Gene Expression Regulation, Neoplastic; Mice; MicroRNAs; 03 medical and health sciences; Urinary Bladder Neoplasms; Cell Movement; Cell Line, Tumor; Disease Progression; Animals; Humans; RNA; Neoplasm Invasiveness; SOXD Transcription Factors; Cell Proliferation; Signal Transduction

AbstractObjectivesTo reveal the role of circular RNA (circRNA) DOCK1 (circDOCK1) as a potential biomarker and therapeutic target and its competing endogenous RNA mechanism in bladder carcinoma (BC).MethodsThe next‐generation sequencing (NGS) technology was introduced to screen the circRNA expression profiles of BC using microarray. qPCR and Western blots assay were employed to measure the gene expression in different groups. Cell counting kit‐8, EdU and transwell assays were applied to detect the cell viability, proliferation and migration potential, respectively. Luciferase reporter assay was used to test the binds between hsa‐miR‐132‐3p/Sox5. Xenografted tumour growth of nude mice was performed to test the role of circDOCK1 in vivo.ResultsCircDOCK1 was upregulated in BC tissues and cell lines. Repression of circDOCK1 reduced cell viability, inhibited cell proliferation and curbed the cell migration potential of BC cell. CircDOCK1 played its role via regulation of circDOCK1/hsa‐miR‐132‐3p/Sox5 pathway in BC cells. Suppression circDOCK1 inhibited the tumour growth in vivo.ConclusionIn this study, we revealed that circDOCK1 affected the progression of BC via modulation of circDOCK1/hsa‐miR‐132‐3p/Sox5 pathway both in vitro and in vivo and providing a potential biomarker and therapeutic targets for BC.