Graft infusion of adipose‐derived mesenchymal stromal cells to prevent rejection in experimental intestinal transplantation: A feasibility study

• DOI: 10.1111/ctr.14226
• Publication Date: 2021-01-19T23:36:46Z
• Authors: Ane M. Andres; Pablo Stringa; Paloma Talayero; Monica Santamaria; Mariano García‐Ar...; Soledad García Gó...; Carlota Largo‐Ara...; Rosa M. Aras‐Lopez; Maria Teresa Vall...; Laura Guerra Past...; Luz Vega; Jose Luis Encinas; Manuel Lopez‐Sant...; Francisco Hernánd...
• Keywords: Immunosuppression

Mesenchymal stromal cells (MSC) have been proposed as a promising complement to standard immunosuppression in solid organ transplantation because of their immunomodulatory properties. The present work addresses the role adipose-derived MSC (Ad-MSC) an experimental model acute rejection small bowel (SBT).Heterotopic allogeneic SBT was performed. A single dose 1.5x106 Ad-MSC intra-arterially delivered just before graft reperfusion. Animals were divided into CONTROL (CTRL), CONTROL+Ad-MSC (CTRL_MSC), tacrolimus (TAC), and TAC+Ad-MSC (TAC_MSC) groups. Each groups subdivided autologous third-party groups.Rejection rate severity similar MSC-treated untreated animals. CTRL_MSC animals showed decrease macrophages, T-cell (CD4, CD8, Foxp3 subsets) B-cell counts compared with CTRL, this attenuated TAC_MSC Pro- anti-inflammatory cytokines some chemokines growth factors increased animals, especially group, whereas milder changes seen TAC groups.Ad-MSC did not prevent when administered However, they effects that could be relevant for longer-term outcome. Interference between should addressed further studies.