Low‐gainer diet‐induced obese microbiota transplanted mice exhibit increased fighting

• DOI: 10.1111/cts.13906
• Publication Date: 2024-08-30T10:39:35Z
• Authors: Caroline M. Junke...; Yan Hui; Tanja Maria Stent...; Malene Klug‐Dambmann; Yi Liu; Line Fisker Zacha...; Lars Hestbjerg Ha...; Antonios Aslampal...; Maria Kiersgaard; Dennis Sandris Ni...; Axel Kornerup Hansen; Lukasz Krych
• Keywords: Male; Leptin; RM1-950; Fecal Microbiota Transplantation; Diet, High-Fat; Weight Gain; Article; Gastrointestinal Microbiome; Mice, Inbred C57BL; Mice; Disease Models, Animal; Feces; Animals; Therapeutics. Pharmacology; Obesity; Public aspects of medicine; RA1-1270

Weight gain variation is a great challenge in diet-induced obesity studies since low-gainer animals are of limited experimental value. The inbred C57BL/6 (B6) mice frequently used models due to their genetic homogeneity and susceptibility (DIO). aim this study investigate if the gut microbiota (GM) influences fraction low weight gainers DIO studies. A total 100 male B6 (donor population) were fed high-fat diet for 14 weeks divided into groups high gainer (HG) (LG) based on gain. Subsequently, fecal matter transplantation (FMT) was done germ-free with GM from HG LG donors (FMT population). (13.35 ± 2.5 g) (25.52 2.0 identified by week 1 12. Interestingly, start (20.36 1.4 (21.59 0.7 differed significantly. Transplanting or resulted significant differences between LG, as well serum leptin levels epididymal fat pad weight. clear LG-specific composition could not be distinguished 16S rRNA gene amplicon sequencing. Surprisingly, significantly more fighting recorded both donor populations when transplanted mice. phenotypes transferred increased group suggests only that tendency fight can FMT these mice, but also should prevented minimize number animals.