Myofibroblast stroma differentiation in infiltrative basal cell carcinoma is accompanied by regulatory T‐cells

• DOI: 10.1111/cup.14381
• Publication Date: 2022-12-23T13:32:50Z
• Authors: Julia Maria Ressler; Nina Zila; Ana Korosec; Josef Yu; Rita Silmbrod; Victoria Bachmayr; Julia Tittes; Johanna Strobl; Beate Maria Licht...; Christoph Hoeller; Peter Petzelbauer
• Keywords: Myofibroblast; CD68; Trichoepithelioma

The implications of infiltrative compared to non-infiltrative growth cutaneous basal cell carcinoma (BCC) on the tumor stroma and immune landscape are unknown. This is clinical importance, because BCCs, in contrast other BCC subtypes, more likely relapse after surgery radiotherapy.This descriptive cross-sectional study analyzed 38 BCCs collected from 2018 2021. In first cohort (n = 28), cells were characterized by immunohistochemistry multiplex immunofluorescence staining for CD3, CD8, CD68, Foxp3, α-SMA protein expression. second 10) with matched characteristics (age, sex, location, subtype), inflammatory parameters, including TGF-β1, TGF-β2, ACTA2, IL-10, IL-12A, quantified via RT-qPCR isolating mRNA tissue samples perilesional skin.Infiltrative showed significantly increased levels expression fibroblasts (p 0.0001) higher Foxp3+ 0.0023) CD3+ 0.0443) T-cells BCCs. 0.0171) regulatory 0.0026) α-SMA-positive stroma, whereas CD8+ 0.1329) CD68+ myeloid 0.2337) not affected. TGF-β1 TGF-β2 correlated ACTA2/α-SMA 0.020, p 0.005).Infiltrative shows a myofibroblastic differentiation accompanied an immunosuppressive microenvironment.